Efficiency of anchored and non-anchored ISSR markers to estimate genetic diversity among bread wheat cultivars

Authors

  • Alireza Tarinejad Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran, Postal code: 53751-71379.
  • Koroush Shams Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran, Postal code: 53751-71379.
  • Omid Sofalian Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran.
  • Rana Valizadeh Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran, Postal code: 53751-71379.
Abstract:

 DNA markers are integrally connected to the success of molecular breeding and are fundamentally required by breeders to be able to, a. identify new gene sources in the available biodiversity, b. select parents in order to increase heterosis, c. decrease the number of backcross generations for gene introgression breeding programs, and d. carry out marker-assisted selection (MAS). The present research was conducted to examine the efficiency of anchored and non-anchored ISSR molecular markers for grouping 20 bread wheat cultivars introduced in a cold and moderate area of Iran. The results showed an average polymorphism of 82.69% and 75% for anchored and non-anchored ISSR markers, respectively. This result indicates that the anchored ISSR markers have a higher rate of efficiency compared to non-anchored ISSR markers. Therefore, simultaneous grouping of cultivars with the use of anchored and non-anchored ISSR markers can differentiate the cultivars of cold and moderate regions. When anchored and non-anchored ISSR markers were simultaneously applied for cluster analysis and grouping of the individuals, the pattern of genetic diversity aligned with the pattern of geographical distribution, and the cultivars attributed to identical geographical regions were allocated in one group. Based on applied clustering with two anchored and non-anchored markers, it was concluded that Pishtaz and Mahdavi cultivars had less genetic similarity compared to other cultivars. 

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Genetic Diversity of Bread Wheat (Triticum aestivum L.) Genotypes Using RAPD and ISSR Molecular Markers

The importance of grain cultivation especially wheat is obvious in terms of providing human and animal food and its impact on the economy of human societies. The reduction of genetic diversity in cultivars prevents increasing yields in line with rising demand and consumption. Therefore, it is necessary to improve the compatibility of them and increase their genetic extent. In the current, the g...

full text

Assessment of Genetic Diversity of Bread Wheat (Triticum aestivum L.) Cultivars using Microsatellite Markers

Determining the level of genetic diversity and relationships among genotypes is foundation for identifying the appropriate parents in different breeding objects. In present study, the genetic diversity of 22 wheat cultivars were tested using 22 pairs of SSR primers. Out of 24 detected alleles, 11 were able to show desirable polymorphism. In average 2.18 alleles per locus were generated, in whic...

full text

A comparative analysis of ISSR and RAPD markers for studying genetic diversity in Iranian pistachio cultivars

Pistachio is one of the most important horticultural products of Iran. The best way to get the maximum yield is to have genetically pure and monotonous gardens. Thereby, study of genetic variation and providing genetic identifications, make this possible to have homogenous gardens with high performance genotypes. In the present study, genetic diversity of 19 Iranian pistachio cultivars was asse...

full text

Study of genetic diversity in bread wheat germplasm using nitrogen uptake and nitrogen use efficiency characteristics

In order to evaluate the traits related to nitrogen utilization and to identify the superior genotypes, 33 bread wheat landraces along three check cultivars of Chamran and Koohdasht (from Iran) and Gobustan (from the Republic of Azerbaijan) were studied in simple lattice statistical design with two replications under two treatments of non-usage and application of 200 kg/ha ammonium nitrate fert...

full text

Evaluation of Genetic Diversity among Some Common Fig Using RAPD and ISSR Molecular Markers

The common fig (Ficus carica L.), one of the most important fruit species, belongs to Moraceae family and is widely distributed in Iran. In this study, genetic variations among some genotypes of common fig collected from six different regions of Ilam province (Iran) were evaluated based on RAPD and ISSR markers. A total of 73 and 29 alleles were produced by 14 RAPD (with their sizes ranging fro...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 4  issue 2

pages  1- 10

publication date 2015-10-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023